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Category Press
Publish Date 10/21/2004
Author Joe Frasca, Vice President, Sales & Marketing
Title Real Time Q-PCR Now Available at LA Testing!
Introduction Clear your mold projects within 24 Hours. No need to wait 7-10 days for Culture results.

Real Time Q-PCR

QPCR - Water Damage Packages
 
QPCR-Water Damage 20     24 Hr Results - $480     48 Hr Results - $295
The following 20 organisms are commonly associated with water damaged environments.
 
Stachybotrys chartarum
Ulocladium botrytis
Aspergillus nidulans
Chaetomium globosum
Aspergillus versicolor
Penicillium expansum
Alternaria alternata
Acremonium strictum
Penicillium aurantiogriseum
Aspergillus flavus
Cladosporium cladosporioides
Penicillium brevicompactum
Penicillium chrysogenum
Aspergillus niger
Cladosporium sphaerospermum
Aspergillus ustus
Aspergillus fumigatus
Aspergillus ochraceus
Trichoderma viride
Paecilomyces variotii
 
QPCR- Water Damage 15     24 Hr Results - $360     48 Hr Results - $240
The following subset of 15 organisms is commonly associated with water damaged environments.
 
Stachybotrys chartarum
Ulocladium botrytis
Penicillium chrysogenum
Aspergillus niger
Aspergillus versicolor
Penicillium expansum
Aspergillus fumigatus
Aspergillus ochraceus
Penicillium aurantiogriseum
Aspergillus flavus
Chaetomium globosum
Penicillium brevicompactum
Acremonium strictum
Aspergillus ustus
Aspergillus nidulans
 
QPCR- Water Damage 10     24 Hr Results - $270     48 Hr Results - $180
The following subset of 10 organisms is commonly associated with water damaged environments.
 
Stachybotrys chartarum
Chaetomium globosum
Penicillium chrysogenum
Penicillium expansum
Aspergillus versicolor
Acremonium strictum
Aspergillus fumigatus
Aspergillus flavus
Penicillium aurantiogriseum
Ulocladium botrytis
 
QPCR-Water Damage 5     24 Hr Results - $150     48 Hr Results - $100
The following subset of 5 organisms is commonly associated with water damaged environments.
 
Stachybotrys chartarum
Penicillium chrysogenum
Penicillium aurantiogriseum
Aspergillus versicolor
Aspergillus fumigatus
 
QPCR - Stachybotrys Package
 
QPCR - Stachybotrys 2     24 Hr Results - $110     48 Hr Results - $75
Stachybotrys chartarum
Stachybotrys (Memnoniella) echinata
 
QPCR - Aspergillus Packages

QPCR - Aspergillus 10     24 Hr Results - $270     48 Hr Results - $180
Aspergillus fumigatus
Aspergillus niger
Aspergillus ochraceus
Aspergillus sydowii
Aspergillus versicolor
Aspergillus ustus
Emericella (A.) nidulans
Eurotium (A.) amstelodami
Aspergillus flavus
Aspergillus parasiticus
 
QPCR - Aspergillus 5     24 Hr Results - $150     48 Hr Results - $100
Aspergillus fumigatus
Aspergillus flavus
Aspergillus parasiticus
Aspergillus versicolor
Aspergillus ochraceus
 
QPCR - Penicillium Packages
 
QPCR - Penicillium 10     24 Hr Results - $270     48 Hr Results - $180
Penicillium chrysogenum
Penicillium brevicompactum
Penicillium aurantiogriseum
Penicillium crustosum
Penicillium expansum
Penicillium corylophilum
Penicillium citrinum
Penicillium fellutanum
Penicillium roquefortii
Penicillium simplicissimum
 
QPCR - Penicillium 5     24 Hr Results - $150     48 Hr Results - $100
Penicillium chrysogenum
Penicillium aurantiogriseum
Penicillium roquefortii
Penicillium expansum
Penicillium citrinum
 
QPCR - Legionella Packages
 
Legionella 4-PCR + Culture w/ presence results  24- 48 Hr Results - $205
Legionella pneumophila, Legionella micdadei, Legionella sainthelensi/cincinnatiensis, Legionella maceachernii
 
We are able to detect the presence/absence of the following 4 species of Legionella by PCR. Quantification is determined by culture if necessary.  You must collect a minimum of 200 mL of water for this test. 100 mL is used for the PCR analysis, 100 mL for the culture if necessary.
 
Legionella 1-PCR + Culture w/ presence results 24- 48 Hr Results - $125
Legionella pneumophila, Legionella micdadei, Legionella sainthelensi/cincinnatiensis, Legionella maceachernii
 
Choose only one of the following Legionella species for PCR analysis. Quantification is determined by culture if necessary.  You must collect a minimum of 200 mL of water for this test. 100 mL is used for the PCR analysis, 100 mL for the culture if necessary.
 
Create Your Own QPCR Package
24 Hr Results - $110    48 Hr Results - $75
 
Choose from the following 34 organisms to create your own package of species. More species will continue to be added over time and with client interest.
 
Acremonium strictum
Alternaria alternata
Aspergillus amstelodami
Aspergillus flavus
Aspergillus fumigatus
Aspergillus niger
Aspergillus nidulans
Aspergillus ochraceus
Aspergillus parasiticus
Aspergillus sydowii
Aspergillus ustus
Aspergillus versicolor
Aurobasidium pullulans
Bacteroides fragillis
Chaetomium globosum
Cladosporium cladosporioides
Cladosporium sphaerospermum
Enterococcus faecalis
Paecilomyces varioti
Penicillium citrinum
Penicillium aurantiogriseum
Penicillium brevicompactum
Penicillium chrysogenum
Penicillium citrinum
Penicillium corylophilum
Penicillium crustosum
Penicillium expansum
Penicillium fellutanum
Penicillium roquefortii
Penicillium simplicissimum
Stachybotrys chartarum
Stachybotrys echinata
Ulocladium botrytis
Trichoderma viride /koningii

Real-Time PCR Sampling Guide

Air Samples
Obtain a 3-piece PCR air/dust-sampling cassette from LA Testing
Remove the upper (blue) and lower (red) plugs of the cassette.
Attach a vacuum pump to the cassette through the lower opening.
Sample as much air as desired through the upper opening. [ There is no upper limit to sampling time.]
Record the VOLUME of air sampled and ship cassette to LA Testing. No refrigeration is needed.

Dust Samples
Obtain a 3-piece PCR air/dust-sampling cassette from LA Testing
Remove the upper (blue) and lower (red) plugs of the cassette.
Attach a small piece of tubing to the upper opening. Cut a 45 degree angle at the end of the tubing.
Attach a vacuum pump to the cassette through the lower opening.
Begin collecting dust through the upper tubing. [ There is no upper limit to sampling time.]
Ship the cassette to LA Testing. No refrigeration is needed.

Swab Samples
Obtain a sterile swab from LA Testing to collect specimen.
Wearing gloves, remove swab from packaging material.
Swab the desired area thoroughly, rolling the swab lightly back and forth over sampling area. Be sure to record the AREA sampled.
Insert the swab into the tube and firmly close cap. Ship the swab to LA Testing. No refrigeration is needed.

Bulk Samples
Obtain a sterile swab and follow directions as described above for swabs.
Alternatively, you may submit a piece of the bulk sample for analysis.

Water/Liquid Samples
All water samples should be taken in sterile sampling containers, which our laboratory can provide upon request.
Fill container with 100 ml of water, or as much water as possible and seal securely to prevent leakage during transport.
Transport sample using ICE PACKS to LA Testing.

Culture Plates 
If you are interested in fast speciation of your culturable (viable) molds in your samples, you may combine PCR with culture analysis. Simply ask the laboratory to hold your cultures for follow-up analysis and request the desired PCR organism package.